Journal: Cellular & molecular biology letters

Article Title: Reciprocal negative feedback between Prrx1 and miR-140-3p regulates rapid chondrogenesis in the regenerating antler.

doi: 10.1186/s11658-024-00573-x

Figure Lengend Snippet: Fig. 4 Characteristics of tissue layers of the antler growth center and isolated RM cells. A Immunofluorescence assay of CD73, CD90, Nestin, CD34, and Prrx1 (red color) in the RM, PC, and CA layers, respectively. B Third passage of cultured cells from the RM layer were identified using mesenchymal stem cell markers: CD73 and CD90 (red), Nestin and CD34 (green), respectively; nuclei were stained blue with DAPI. C Chondrogenic differentiation of RM cells, Alcian blue, and Col II immunofluorescence staining. D Osteogenic differentiation of RM cells with Alizarin red staining. E Adipogenic differentiation of RM cells with Oil Red O staining (scale bars, 125 μm and magnification ×200)

Article Snippet: The cells and tissues were fixed, permeabilized, blocked, and incubated overnight with primary antibodies: CD90 (1:300, proteintech, USA, 66766-1-lg), CD73 (1:1000, proteintech, USA, 12231- 1-AP), Nestin (1:300, BIOSS, China, bs-0008R), CD34 (1:300, proteintech, USA, 14486- 1-AP), and Prrx1 (1:200, Absin, China, abs134576).

Techniques: Isolation, Immunofluorescence, Cell Culture, Staining

Journal: Arquivos Brasileiros de Cardiologia

Article Title: Effects of Chronic Exercise on Endothelial Progenitor Cells and Microparticles in Professional Runners

doi: 10.5935/abc.20170022

Figure Lengend Snippet: Box-plots showing the percentage of circulating endothelial progenitor cells (EPCs) determined by flow-cytometry. Higher percentage of CD34+/KDR+ EPCs (A) (p=0.038 vs. controls, Mann-Whitney U test), as well as CD133+/KDR+ EPCs (p=0.018 vs. controls, Mann-Whitney U test) (B) were found in athletes. No differences were observed between groups for CD133+/CD34+ (p=0.51) (C).

Article Snippet: Fluorescently labeled mouse anti-human antibodies were used for EPCs (CD34 FITC, BD Biosciences, USA; CD133 APC, Miltenyi Biotec, USA; KDR PE, R&D Systems, USA), PMPs (CD42 FITC and CD31 PE, BD Biosciences, USA) and EMPs (CD51 FITC, BD Biosciences).

Techniques: Flow Cytometry, MANN-WHITNEY

Journal: Journal of cancer research and therapeutics

Article Title: Syk expression in non-small-cell lung cancer and its relation with angiogenesis.

doi: 10.4103/0973-1482.154082

Figure Lengend Snippet: Figure 2: CD34 immunostaining locates in microvascular endothelial cells with brown granular in tumor tissue SABC × 200

Article Snippet: Streptavidin‐biotin complex (SABC) kit, CD34 antibody, and DAB chromogenic reagent were from Wuhan Boster Biotechnology Company.

Techniques: Immunostaining

Journal: Experimental & Molecular Medicine

Article Title: Generation and characterization of integration-free induced pluripotent stem cells from patients with autoimmune disease

doi: 10.1038/emm.2016.27

Figure Lengend Snippet: Differentiation of autoimmune disease (AID)-specific induced pluripotent stem cells (iPSCs) into various hematopoietic lineages. ( a ) A schematic diagram of the protocol used to obtain multipotent hematopoietic stem cells (HSCs) from patient-specific iPSCs obtained from patients with ankylosing spondylitis (AS), Sjögren syndrome (SS) and systemic lupus erythematosus (SLE). ( b ) Fluorescence-activated cell sorting (FACS) analysis of HSCs derived from patients with specific AIDs. After 14 days of differentiation, cells were analyzed by flow cytometry for the expression of CD34, CD43 and CD45. The data are presented as mean (s.e.m.) of three individual experiments. ( c ) Colony-forming unit (CFU) assay of AID-specific iPSC-derived HSCs. The left panel shows the morphology of the CFUs, and the right panel shows the number of CFUs per 2 × 10 4 cells ( d ). CFU-E, CFU-erythroid; CFU-G, CFU-granulocyte; CFU-M, CFU-macrophage; CFU-GM, CFU-granulocyte and macrophage. The right panel shows the average CFU values per 2 × 10 4 cells at day 21.

Article Snippet: The cells were labeled with antibodies against CD43-PE, CD45-APC, CD34-APC, CD73-PE and CD90-PE (Miltenyl Biotec, Cologne, Germany) at 4 °C for 30 min, washed twice with staining buffer and then analyzed on a BD Accuri C6 flow cytometer (BD Biosciences, Bedford, MA, USA) according to the manufacturer's instructions.

Techniques: Fluorescence, FACS, Derivative Assay, Flow Cytometry, Expressing, Colony-forming Unit Assay