Journal: Brain and Behavior

Article Title: Electrical stimulation improved cognitive deficits associated with traumatic brain injury in rats

doi: 10.1002/brb3.667

Figure Lengend Snippet: Flow cytometry detection of endothelial progenitor cells ( EPC s) in peripheral blood of rats before the traumatic brain injury ( TBI ) (0 day), and 1, 3, 7, 14, 21, 28 days after TBI . They were marked by CD 34 and CD 133. The stress of TBI mobilized the EPC s at 1 day after TBI in N‐ ES group and ES group. Electrical stimulation treatment increased EPC s numbers in peripheral blood from 3 to 21 days after TBI . n = 6/group; * p < .05 ES group versus N‐ ES group; # p < .05 N‐ ES group and ES group versus Sham group

Article Snippet: CD34 + cells in brain tissue were detected using a CD34 antibody (R and D Systems Cat# AF4117 Lot# RRID:AB_2074613 ) as recommended by the manufacturer.

Techniques: Flow Cytometry

Journal: bioRxiv

Article Title: Propranolol reduces sarcoma growth and enhances the response to anti-CTLA4 therapy by modulating the tumor microenvironment

doi: 10.1101/2021.03.11.434711

Figure Lengend Snippet: Propranolol treatment reduces MCA205 tumor angiogenesis. A and B , Quantification of angiogenic marker Vegfa (A) and Kdr (B) gene expression in whole tumor RNA from control mice and propranolol (PRO) treated mice by qRT-PCR (n=5). C , Representative CD34 (brown) IHC staining of MCA205 tumor tissue from control mice or propranolol treated mice; hematoxylin counterstain; scale bar 100µm (left panels), and 50µm (right panels). D , Dot plot showing quantification of CD34 staining by percentages of DAB+ area in 9 control mice and 11 propranolol treated mice. *p<0.05, **p<0.01 according to multiple t test with Bonferroni correction. Mean ± SEM are depicted.

Article Snippet: For CD34 immunostaining, 10 mM citrate buffer, Ph antigen retrieval, and rat anti-mouse CD34 (1:400, MEC 14.7, Novus Biologicals) were used.

Techniques: Marker, Gene Expression, Control, Quantitative RT-PCR, Immunohistochemistry, Staining

Journal: bioRxiv

Article Title: Propranolol reduces sarcoma growth and enhances the response to anti-CTLA4 therapy by modulating the tumor microenvironment

doi: 10.1101/2021.03.11.434711

Figure Lengend Snippet: Propranolol combined with anti CTLA4 increases the number of intratumoral CD8+ T cells, reduces tumor angiogenesis, and provides long lasting immune memory against MCA205 cancer cells. A and C , Representative CD8 ( A ) and CD34 ( C ) IHC staining of MCA205 tumor tissues; hematoxylin counterstain; scale bar 100µm (left panels), and 50µm (right panels). B and D , Dot plot showing quantification of CD8 ( B ), and CD34 staining ( D ). Splenocytes from cured mice were re-stimulated ex vivo with MCA205 cancer cells for 24 hours, and intracellular cytokine expressions were quantified by flow cytometry. E , Representative flow cytometry dot plots showing the expression of TNFα and IFNγ on CD4+ T cells (upper panels) or CD8+ T cells (lower panels) with memory phenotype from naïve mice (left panels) or anti-CTLA4+PRO treated mice that had shown complete tumor regression(right panels). F , Percentages of tumor reactive T cells (IFNy+ or TNFa+) with memory phenotype (CD44hi) among CD8+ or CD4+ T cells after 24-hour ex vivo re-stimulation. *p<0.05 according to multiple t test with Bonferroni correction. Mean ± SEM are depicted.

Article Snippet: For CD34 immunostaining, 10 mM citrate buffer, Ph antigen retrieval, and rat anti-mouse CD34 (1:400, MEC 14.7, Novus Biologicals) were used.

Techniques: Immunohistochemistry, Staining, Ex Vivo, Flow Cytometry, Expressing

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Delivery of Human Stromal Vascular Fraction Cells on Nanofibrillar Scaffolds for Treatment of Peripheral Arterial Disease

doi: 10.3389/fbioe.2020.00689

Figure Lengend Snippet: Flow cytometric analysis of human stromal vascular fraction (SVF) cells. (A) Representative flow cytometric plot depicts subpopulations of cells based on the expression of CD31 and CD45. (B) Flow cytometric analysis of the CD31 – /CD45 – subpopulation showing that the majority of the cells are CD34 + . (C) Mean cell fraction data among six independent donor SVF extractions.

Article Snippet: To visualize the persistence of human SVF cells, tissue sections were immunofluorescently stained with antibodies directed against human-specific nuclear matrix antigen (Millipore), human-specific CD34 (Novus Biologicals), and human-specific CD31 (Dako).

Techniques: Expressing

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Delivery of Human Stromal Vascular Fraction Cells on Nanofibrillar Scaffolds for Treatment of Peripheral Arterial Disease

doi: 10.3389/fbioe.2020.00689

Figure Lengend Snippet: Characterization of human SVF cells on aligned nanofibrillar collagen scaffolds. (A) Scanning electron microscopy image depicts nanofibrils arranged in parallel along the direction of the arrows. Confocal images show CD34 (B) , CD31 (C) , and CD105 (D) protein expression among adherent SVF cells on aligned nanofibrillar scaffolds after 1 day of attachment. Scale bars: 2 μm (A) ; 50 μm (B–D) .

Article Snippet: To visualize the persistence of human SVF cells, tissue sections were immunofluorescently stained with antibodies directed against human-specific nuclear matrix antigen (Millipore), human-specific CD34 (Novus Biologicals), and human-specific CD31 (Dako).

Techniques: Electron Microscopy, Expressing

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Delivery of Human Stromal Vascular Fraction Cells on Nanofibrillar Scaffolds for Treatment of Peripheral Arterial Disease

doi: 10.3389/fbioe.2020.00689

Figure Lengend Snippet: Immunofluorescence staining of retained human SVF adjacent to the site of cell-seeded scaffold transplantation. (A) Human SVF are visualized using human specific nuclear matrix antigen (NuMA). Immunofluorescence imaging of human-specific antibodies targeting CD34 (B) and CD31 (C) . Scale bar: 50 μm (A) , 100 μm (B,C) .

Article Snippet: To visualize the persistence of human SVF cells, tissue sections were immunofluorescently stained with antibodies directed against human-specific nuclear matrix antigen (Millipore), human-specific CD34 (Novus Biologicals), and human-specific CD31 (Dako).

Techniques: Immunofluorescence, Staining, Transplantation Assay, Imaging

Journal: Journal of Nanobiotechnology

Article Title: Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration

doi: 10.1186/s12951-023-02125-5

Figure Lengend Snippet: Characterization of hUCMSCs. A Primary hUCMSCs were spindle-shaped under light microscopy. B Flow cytometry analysis showed that 98.5% of cells expressed CD29, 97.4% expressed CD44, 98.6% of cells expressed CD90, and 98.1% of cells expressed CD105. Meanwhile, only 0.48% and 0.28% expressed CD45 and CD34. C Lipid droplets were stained by oil red O after adipogenic differentiation induction of hUCMSCs. Mineralized nodules were stained by Alizarin Red after osteogenic differentiation induction of hUCMSCs. Acid mucopolysaccharide was stained by Alcian Blue after chondrogenic differentiation induction of hUCMSCs

Article Snippet: Briefly, 10 7 hUCMSCs were subjected to analysis for surface markers CD34 (E-AB-F1143D, Elabscience), CD44 (E-AB-F1100D, Elabscience), CD45 (E-AB-F1137D, Elabscience), CD29 (E-AB-F1049D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and CD105 (E-AB-F1310D, Elabscience) by flow cytometry following the manufacturer’s protocol.

Techniques: Light Microscopy, Flow Cytometry, Staining

Journal: Biomaterials

Article Title: The effect of stromal cell-derived factor-1α/heparin coating of biodegradable vascular grafts on the recruitment of both endothelial and smooth muscle progenitor cells for accelerated regeneration.

doi: 10.1016/j.biomaterials.2012.07.042

Figure Lengend Snippet: Fig. 3. En face immunostaining for CD31, CD34, CD45 and CXCR7 of the explanted grafts at 1-week after implantation. (AeI) En face immunostaining for CD31 (green) and CD34 (red) of proximal, middle and distal portions of untreated grafts (AeC), heparin-treated grafts (DeF) and heparin-SDF-1a-treated grafts (GeI). (JeL) En face immunostaining for CD45 (green) and CXCR7 (red) of the untreated control grafts (J), heparin-treated grafts (K) and heparin-SDF-1a-treated grafts (L). Arrows indicate CD45þ cells. Nuclei were stained by DAPI (blue). Scale bar ¼ 40 mm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Immunohistochemical staining was used to analyze the tissue sections with the following primary antibodies: SM-MHC (sc-79079, goat, Santa Cruz Biotechnology Inc.), CNN1 (1806-1, rabbit, Epitomics, Inc.), SMA (1184-1, rabbit, Epitomics, Inc.), elastin (ab21610, rabbit, Abcam Inc.), Collagen type I (ab34710, rabbit, Abcam Inc.), CD31 (550300, mouse, BD Biosciences), CD34 (AF4117, rat, R&D), CD133 (ab19898, rabbit, Abcam Inc.), CD45 (05-1410, mouse, Millipore), CXCR7 (51024-1-AP, rabbit, Proteintech Group), CXCR4 (ab2074, rabbit, Abcam Inc.), CD68 (MCA341R, mouse, AbD Serotec) and CD3 (ab8879, mouse, Abcam Inc.).

Techniques: Immunostaining, Control, Staining

Journal: Biomaterials

Article Title: The effect of stromal cell-derived factor-1α/heparin coating of biodegradable vascular grafts on the recruitment of both endothelial and smooth muscle progenitor cells for accelerated regeneration.

doi: 10.1016/j.biomaterials.2012.07.042

Figure Lengend Snippet: Fig. 4. En face immunostaining for CD31 (green) and CD34 (red) of the explanted grafts at 2-week (AeI) and 4-week (JeR) after implantation. Nuclei were stained by DAPI (blue). Scale bar ¼ 40 mm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Immunohistochemical staining was used to analyze the tissue sections with the following primary antibodies: SM-MHC (sc-79079, goat, Santa Cruz Biotechnology Inc.), CNN1 (1806-1, rabbit, Epitomics, Inc.), SMA (1184-1, rabbit, Epitomics, Inc.), elastin (ab21610, rabbit, Abcam Inc.), Collagen type I (ab34710, rabbit, Abcam Inc.), CD31 (550300, mouse, BD Biosciences), CD34 (AF4117, rat, R&D), CD133 (ab19898, rabbit, Abcam Inc.), CD45 (05-1410, mouse, Millipore), CXCR7 (51024-1-AP, rabbit, Proteintech Group), CXCR4 (ab2074, rabbit, Abcam Inc.), CD68 (MCA341R, mouse, AbD Serotec) and CD3 (ab8879, mouse, Abcam Inc.).

Techniques: Immunostaining, Staining